Lick n’ Light Lollipop and Candy Safety



Information
Our candies glow using a natural process called bioluminescence. The same process that fireflies, glowworms, shrimp, squid and many other marine animals use to make light.

Bioluminescence is a naturally occurring process that utilizes protein enzymes to add oxygen to a small anti-oxidant molecule called Coelenterazine. Just like when animals “burn” sugar for energy, bioluminescence “burns” an anti-oxidant for energy in the form of light. This is considered “cold-burning” since no heat is created.



Click the link if you would like to see a short video on where Lick n’ Light Chemistry comes from:

The Video



Safety Testing Summary:

Name of Test Description Result
Absorption to bloodstream This tests the amount of Coelenterazine and Coelenteramide absorbed into the bloodstream after eating a large amount of pure glowing chemistry. A large dose is injected into the bloodstream and is checked over time to make a curve, then an oral dose is given and checked at the same intervals. Two test species. Less then 0.5% of chemistry is orally absorbed. None detected at 24 hours. (Average Lollipop Dose is less then 1 mg)
LD 50 This tests the amount of substance required to kill 50% of the tested population. We performed 6 tests on Coelenterazine, Coelentermide, Coelentermine, Renilla Luciferase, Renilla Luciferase + Coelenterazine, and Gaussia Luciferase.* (None died, none even sick at this oral level) The LD 50 on all tests was found to be greater than 5g/kg. (2.5g/kg is the maximum suggested by FDA and OECD)
Ames Test Coelenterazine (CAS# 55779-48-1) This test applies high doses of the test substance to specialized bacteria on plates to determine if they cause mutations changes to the bacteria colony shape are observed visually. No mutations observed.
Reverse Ames Coelenterazine and the reaction product, Coelenteramide is applied in high concentrations to specialized strains of bacteria used just for this purpose, and observed if they start producing colonies that are readily identified as forming mutation or changes to the DNA. Negative
Negative
Chromosomal Aberration This tests for mutagens by using live cells in a culture dish and adding various doses of the test substances (Coelenterazine and Coelenteramide) and looking for mutations to the chromosomes by staining and counting the breaks in Chinese Hamster Ovarian Cells in tissue culture. Negative
Bone Marrow This tests for bone marrow changes by feeding or injecting the substance (Coelenterazine) and checking rapidly dividing Bone Marrow cells and looking for changes to their nuclei and cellular changes. Negative
50x Product Testing This tests Lick n’ Light Chemistry at 50 times their normal concentration in Lollipop sugars and Cake Frosting Cream to test animals for a 14-day period. No animals showed any signs of toxicity after two weeks daily consumption. The LD 50 were estimates only. No adverse affects
LD50 >15 g/kg
LD50 >10 g/kg


* If you notice in the graphical NanoLight projector image (above) you will see that Coelenterazine (the anti-oxidant molecule) is converted to Coelenteramide after the light is produced. Toxicology tests were performed on both compounds because the consumer would actually be consuming both compounds after light is emitted.



These tests were performed under OECD 425 and FDA approved format in licensed, audited, and inspected facilities. For specifics, take a look at our detailed safety data page or if you have any questions about these tests or would like to see a copy of the original testing done, please contact us at safety@licknlight.com



Human Foods containing Lick n’ Light chemistry

If you have ever gone swimming in the ocean and accidentally swallowed a gulp of seawater, you have probably ingested some of our chemistry already. Currently there are many marine animals consumed by people that contain the same chemistry as our Lick n’ Light Lollipops. The most popular glowing human food is the Firefly Squid (Watsenia scinillans) mainly caught and consumed in Japan and China.

Sophisticated liquid chromatography and mass spectrographs were performed to compare the amount of Lick n’ Light chemistry in a 100 gram serving of Firefly Squid and was found to be approximately the same dose as found 5 lollipops. Tuna, Sardines, and many canned foods contained identical chemistry however in much lower concentrations (these fish consume other fish that glow and contain Lick n’ Light chemistry in their tissues and organs.

Here is a picture of the Squid Harvest in Japan:

Lick n’ Light Chemistry a useful Anti-oxidant

Scientists have tested and published papers describing the amazing properties of Coelenterazine as an anti-oxidant; to prevent fat rancidity in foods; and cellular protectant against harsh chemical applied to cells which greatly protecting them from oxidative stressors and peroxides.

Coelenterazine has been tested as a possible Liver Transplant Protectant and has been found to reduce cellular swelling in transplanted livers by protecting cellular membranes from peroxides during warming after transplantation. We are currently conducting test to see if Coelenterazine can be used to prevent cellular injury and swelling during heart surgery and kidney transplants.
(Published Data:
Toxicity of Coelenterazine (CTZ) in Liver Preservation Solutions
REF 16 CTZ analogs vitro evaluation of as inhibitors lipid peroxidation
REF 17 CTZ protection
REF 18 Ref 18 CTZ cytoprotection)



Lick n’ Light Chemistry Used in Cancer Imaging, Stem Cell Research, and Drug Discovery.

Please click on www.pubmed.gov and search the following terms:

‘Gaussia Luciferase,’ ‘Coelenterazine,’ and ‘Renilla Luciferase’ for peer reviewed publications. Lick n’ Light Chemistry used for over fifteen years in many research publications to detect cancers, in clinical laboratory tests, and will hopefully soon be used in surgery to prevent injury.

Cancer Imaging
Here are some movies of actual surgery using Lick n’ Light chemistry as an imaging agent. It does not make or require any X-rays, this saves the patient and surgeon exposure to accumulated radiation and makes the anatomy clearly visible so less surgical mishaps occur (“Lumigrams”).

- Lick n’ Light chemistry in Breast Cancer Research
- Lick n’ Light chemistry in Brain Cancer research
- Lick n’ Light chemistry in Diabetes Research

Surgical Imaging
- Lick n’ Light chemistry replaces x-rays
- Lick n’ Light chemistry in intestinal surgery
- Lick n’ Light chemistry in preventing gallbladder surgical injury
- Lick n’ Light chemistry in heart surgery

There is a lot more then we can present here, feel free to contact us for actual test data and reports, and other uses for Lick n’ Light Chemistry and info@licknlight.com for questions.